Royal Bank of Scotland have confirmed the Gloucester branch will close later this year.
The Westgate Street branch will close on Wednesday 1st August.
RBS customers will be able to bank in person at Natwest instead.
The bank, which has previously blamed falling branch business and growing numbers of digital banking customers for shutting sites in recent years, said the move this time largely reflected its decision not to sell its Williams & Glyn business.
It said that by not creating the challenger bank, as it had once been required to do by regulators, it was removing geographical duplication in its branch network.
It meant that the bank, still majority owned by the taxpayer after its 2008 bailout, has announced a total of 569 branch closures since March 2017.
The plans will leave the group with just 105 RBS sites in England and Wales as it looks to build its Natwest brand, which would have 650 high street outlets.
RBS revealed that 109 branches would close this summer in the latest round of cuts – all of them currently within 0.6 miles of another RBS or NatWest branch, it said.